degS [2017-8-11 16:0:3]

two-component sensor kinase for exoenzyme and competence regulation, kinase activity is activated in response to inhibition of flagellar rotation

locus: BSU35500

pI: 5.99

mw: 44.80 kDa

protein length: 385 aa

gene length: 1155 bp

function: regulation of degradative enzyme and genetic competence

product: two-component sensor kinase

essential: no

synonyms: sacU

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 3,645,379 → 3,646,536

Phenotypes of a mutant

• the mutation suppresses the mucoid phenotype of ''[gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]'' or ''[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]'' mutants due to loss of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] phosphorylation and concomitant reduced expression of the ''[gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]-[gene|D66994D077D1382B88FF65075E44C2A31089548F|capC]-[gene|A696434A086A428D411CAF45B37CD8F82AC2503F|capA]-[gene|6B61DCE8D27776EBF942621D3AE90F410FD13F37|capE]'' operon [Pubmed|24296669]

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, autodephosphorylation, phosphorylation of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] on Asp-56 [Pubmed|1901568]

[SW|Domains]

• aa 12 ... 170: unknown DegS domain
• aa 180 ... 248: HisKA_3 domain (autophosphorylation)
• aa 289 ... 384: HATPase_c

Modification

• phosphorylation on Ser-76 [Pubmed|17218307], this stimulates phosphotransfer from His-189 to [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] [Pubmed|21304896], the phosphorylyation is performed by [protein|E0097A00C82136BB0B1FB89FBA177E28A4EC3D54|PrkD] ''in vitro'' [Pubmed|21304896]
• autophosphorylation on His-189

Effectors of protein activity

• [protein|40C1E81BAB04BD1CC98FC57DF25D27DBDFEB5A59|DegQ] (stimulation of autophosphorylation) [Pubmed|21965392]
• inhibition of flagellar rotation stimulates kinase activity [Pubmed|23888912]

[SW|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|degS]-[gene|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]
  description: [Pubmed|1688843]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1688843], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|18502860], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: activation, [Pubmed|23123903], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|24317403], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]

  regulation

  • induced by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|23123903]

Labs working on this gene/protein

• [SW|Tarek Msadek], Institut Pasteur, Paris, France

References

Reviews

• 23927648

Original Publications

• 17850253,12471443,14563871,1459944,1901568,10094672,1688843,16479537,17218307,21304896,21965392,24296669,23888912,25433860